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AssigneeAZ. Agricola T.&T. Produce Di Tiozzo Silvano
InventorsGIANNI BARCACCIA, Silvano Caenazzo Tiozzo
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The present invention relates to leaf chicory (Cichorium intybus subsp. intybus var. foliosum) nuclear recessive male sterile mutants, to a newly identified polymorphic molecular marker for the nuclear recessive male sterile character in this species, to methods for the selection of leaf chicory nuclear recessive male sterile mutants, to methods for the production of seed parent male sterile and pollen donor male fertile inbred lines of leaf chicory, including all cultivated types of radicchio, that are, respectively, homozygous for the nuclear recessive male sterile mutant allele or homozygous for the male fertile wildtype allele, and to methods for the constitution of F1 hybrids that are all heterozygous at the ms locus.
A male sterile mutant of leaf chicory, including Radicchio (Cichorium intybus subsp. intybus var. foliosum), parts or derivatives thereof, wherein the male sterility trait:
A polymorphic genetic marker comprising SEQ ID NO 1, said marker being linked to a locus of nuclear recessive male sterile mutation (ms) in leaf chicory species, including all biotypes of cultivated Radicchio.
A method for the selection of mutant plants of leaf chicory, including all Radicchio cultivated types, that are homozygous or heterozygous for a mutation inducing nuclear recessive male sterility (ms) linked to a polymorphic genetic marker comprising a simple sequence repeat (TC)n in SEQ ID NO 1 or 2 comprising the steps of:
A method for the production of mutant male sterile seed parents of leaf chicory plant materials wherein the male sterility is a nuclear recessive male sterility due to mutation of a nuclear gene (ms) linked to a polymorphic genetic marker comprising a simple sequence repeat (TC)n in SEQ ID NO 1 or 2, comprising the steps of:
A method for the production of mutant F1 hybrids of leaf chicory (Cichorium intybus subsp. intybus var. foliosum), including all cultivated types of Radicchio wherein said hybrids are fertile and heterozygous for a mutation in a gene inducing nuclear recessive male sterility (ms) linked to a polymorphic genetic marker comprising a simple sequence repeat (TC)n in SEQ ID NO 1 or 2 comprising the steps of:
A male sterile mutant of Cichorium intybus subsp. intybus var. foliosum, parts or derivatives thereof, wherein the genotype responsible for male sterility is ascribable to a recessive mutation (ms) of a single nuclear gene tightly linked to a polymorphic molecular marker comprising a microsatellite or simple sequence repeat (SSR) that is a (TC)n repetition, wherein n is an integer ranging between 27 and 33 (SEQ ID NO:
A polymorphic molecular marker comprising SEQ ID NO 1 or consisting of SEQ ID NO 2 that is a (TC)n repetition wherein n is an integer ranging from 25 up to 39 (SEQ ID NO:
A male sterile mutant of a plant, parts or derivatives thereof, wherein the male sterility trait is controlled by a nuclear recessive mutation (ms).
Dependencies are inferred from the claim text.