A process for separating isomers by simulated moving bed (SMB) adsorption, comprising separating the feedstock comprising isomers by SMB adsorption, said SMB comprising many adsorption beds each of which is equipped with grids, each of the grids being equipped with the materials charging and discharging pipeline of the bed, the materials charged into and discharged from SMB at least comprising feedstock, desorbent, extract, and raffinate, the extract being enriched with the target product, characterized in that the extract is used as a flushing liquid and respectively charged to first or second bed at the upstream of the feedstock charging position and to one of second to fourth beds at the downstream of the extract withdrawing position.