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US 2014/0155581 A1Published application
Methods For Making Multimeric Polypeptides
OwnerChangshou Gao, Nazzareno Dimasiinventors
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Abstract
Detailed herein are contiguous, multimeric, multispecific polypeptides, nucleic acids encoding such polypeptides, and methods for making such polypeptides and nucleic acids.
Claims27
A nucleic acid molecule encoding a contiguous, multimeric polypeptide comprising at least two subunits, each of which includes at least a functional domain (FD) and an interaction domain (ID), wherein the nucleic acid molecule comprises:
- i) a nucleic acid portion comprising a nucleotide sequence that encodes a functional domain (FD1) that binds to a first binding site;
- ii) a nucleic acid portion comprising a nucleotide sequence that encodes an interaction domain (ID1);
- iii) a nucleic acid portion comprising a nucleotide sequence that encodes a functional domain (FD2) that binds to a second binding site;
- and
- iv) a nucleic acid portion comprising a nucleotide sequence that encodes an interaction domain (ID2);
- wherein the nucleic acid molecule further comprises at least one nucleic acid portion comprising a nucleotide sequence encoding a polypeptide linker, which polypeptide linker includes at least one protease cleavage site;
- wherein ID1 and ID2 are capable of associating with each other, and wherein the contiguous, multimeric polypeptide is multispecific.
4 more elements
2-4. (canceled)
(canceled)
8-10. (canceled)
A nucleic acid molecule encoding a contiguous polypeptide, wherein the nucleic acid molecule comprises:
- i) a nucleic acid segment comprising a nucleotide sequence that encodes an antibody light chain comprising a variable domain (VL1) and a light constant domain 1;
- ii) a nucleic acid segment comprising a nucleotide sequence that encodes a first polypeptide linker;
- iii) a nucleic acid segment comprising a nucleotide sequence that encodes an antibody heavy chain comprising a variable domain (VH1) and a heavy constant domain 1;
- iv) a nucleic acid segment comprising a nucleotide sequence that encodes a second polypeptide linker;
- v) a nucleic acid segment comprising a nucleotide sequence that encodes an antibody light chain comprising a variable domain (VL2) and a light constant domain 2;
- vi) a nucleic acid segment comprising a nucleotide sequence that encodes a third polypeptide linker;
- and
- vii) a nucleic acid segment comprising a nucleotide sequence that encodes an antibody heavy chain comprising a variable domain (VH2) and a heavy constant domain 2;
- wherein the contiguous polypeptide is a multispecific polypeptide, and wherein each of the three polypeptide linkers comprises at least one protease cleavage site.
6 more elements
23-37. (canceled)
Dependencies are inferred from the claim text.