Microorganisms and methods for the biosynthesis of butadiene
AssigneeGenomatica, Inc.
InventorsMark J. Burk, Anthony P. Burgard, Jun Sun, Robin E. Osterhout, Priti Pharkya
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Abstract
The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.
Claims32
A non-naturally occurring microbial organism, said microbial organism having a butadiene pathway and comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce butadiene, said butadiene pathway comprising converting crotonyl-CoA to crotyl-alcohol using one or more butadiene pathway enzymes, wherein said butadiene pathway enzymes are selected from the group consisting of a crotonyl-CoA reductase (aldehyde forming), a crotonaldehyde reductase (alcohol forming) a crotonyl-CoA hydrolase, synthetase, or transferase, a crotonate reductase, and a crotonyl-CoA reductase (alcohol forming).
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Biosynthesized butadiene.
A process for producing the polymer of 66 comprising chemical or enzymatic conversion of biosynthesized butadiene to produce said polymer.
Dependencies are inferred from the claim text.