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AssigneeSt. Jude Children's Research Hospital
InventorDavid Ellison
Estimating the term…
Immunohistochemical methods and compositions for the typing of molecular subgroups of medulloblastomas are provided. The methods comprise determining a protein expression profile for a sample obtained from a medulloblastoma by detecting expression of GAB 1, filamin A, or at least two biomarker proteins selected from the group consisting of β-catenin, YAP1, GAB1, and filamin A, and typing the medulloblastoma as a WNT pathway tumor, a SHH pathway tumor, or a non-WNT/non-SHH tumor based on this protein expression profile. Kits for typing a medulloblastoma according to these three molecular subgroups are provided. The kits comprise at least two antibodies, wherein each of said antibodies specifically binds to a distinct biomarker protein selected from the group consisting of β-catenin, YAP1, GAB1, and filamin A, and can optionally comprise one or more of instructions for use, reagents for detecting antibody binding to one or more of said biomarker proteins, and one or more positive control samples.
An immunohistochemical method for typing a medulloblastoma as a WNT pathway tumor, a sonic hedgehog (SHH) pathway tumor, or a non-WNT/non-SHH tumor, said method comprising determining a protein expression profile for a sample obtained from said medulloblastoma by detecting expression of β-catenin, YAP1, GAB1, or filamin A, and typing said medulloblastoma as a WNT pathway tumor, a SHH pathway tumor, or a non-WNT/non-SHH tumor based on said protein expression profile.
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An immunohistochemical method for typing a medulloblastoma as a SHH pathway tumor or a non-SHH tumor, said method comprising determining a protein expression profile for a sample obtained from said medulloblastoma by detecting expression of GAB1 and typing said medulloblastoma as a SHH pathway tumor or a non-SHH tumor based on said protein expression profile.
An immunohistochemical method for typing a medulloblastoma as a non-WNT/non-SHH tumor, said method comprising determining a protein expression profile for a sample obtained from said medulloblastoma by detecting expression of filamin A, and typing said medulloblastoma as a non-WNT/non-SHH tumor based on said protein expression profile.
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A kit for typing a medulloblastoma as a WNT pathway tumor, a sonic hedgehog (SHH) pathway tumor, or a non-WNT/non-SHH tumor, said kit comprising at least two antibodies selected from the group consisting of an antibody that specifically binds β-catenin, an antibody that specifically binds YAP1, an antibody that specifically binds GAB1, and an antibody that specifically binds filamin A.
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