Methods and compositions for improved cattle longevity and milk production
AssigneeWisconsin Alumni Research Foundation
InventorsHasan Khatib, Wen Huang
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Abstract
A single nucleotide polymorphic site at position 10793 of the bovine POU1F1 gene is associated with improved longevity and milk product traits. Disclosed are nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods.
Claims25
An isolated nucleic acid molecule comprising a polymorphic site at position 10793 and at least 15 contiguous bases of SEQ ID NO:
- 1 adjacent to the polymorphic site, wherein the nucleic acid molecule comprises an adenine base at position 10793, or a nucleic acid molecule that is fully complementary to the nucleic acid molecule.
A method for detecting single nucleotide polymorphism (SNP) in bovine POU1F1 gene, wherein the PI gene have a nucleic acid sequence of SEQ ID NO:
- 1, the method comprising determining the identity of a nucleotide at position 10793, and comparing the identity to the nucleotide identity at a corresponding position of SEQ ID NO:
- 1.
A method according to claim 16, wherein the gene or a relevant fragment thereof is isolated from the cell via amplification by the polymerase chain reaction (PCR) of genomic DNA of the cell, or by RT-PCR of the mRNA of the cell.
A method for selectively breeding dairy cattles, comprising selecting a bull that is homozygously A at position 10793 of the POU1F1 gene and using its semen for fertilizing a female animal.
Dependencies are inferred from the claim text.