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US 2014/0155290 A1Published application
Host cells comprising alpha 1, 2 mannosidase and culture methods thereof
AssigneeAmgen Inc.
InventorChristopher Kenyon Crowell
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Abstract
Improved host cells and culture methods involving overexpression of MAN1C1 activity to improve protein production are provided.
Claims23
A host cell engineered to overexpress (a) alpha 1,2 mannosidase (MAN1C1) native to the host cell and (b) a glycoprotein of interest.
A method of screening for an inducer of protein production comprising the steps of:
- (a) contacting a host cell with a candidate compound,
- (b) determining expression level of MAN1C1, and
- (c) identifying said candidate compound as an inducer of protein production if the expression level of MAN1C1 increases.
Dependencies are inferred from the claim text.