Detection of a panel of urine DNA markers for HCC screening and disease management

A method of detecting the presence or absence of a cancer in a biological sample of a subject in need thereof, comprising:

• (v) preparing DNA from the biological sample;
• (vi) determining the level of mutation or methylation of one or more genes from a group of genes comprising TP53, CTNNB1, hTERT, RASSF1A, GSTP1, p16, p15 and SFRP-1 from the biological sample of the subject;
• (vii) comparing the level of mutation or methylation of the one or more genes with the level of mutation or methylation of the one or more genes in one or more control samples from subjects known not to have said cancer;
• and
• (viii) detecting the presence or absence of the cancer, with elevated mutation or methylation levels in the one or more genes of the individual as compared to the level of mutation or methylation in the one or more genes in the one or more control samples indicating the presence of the cancer, and the absence of elevated mutation or methylation levels indicating the absence of the cancer. The method claim of 1, wherein the biological sample comprises tissue or body fluid selected from the group consisting of serum, plasma, and urine.