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US 2014/0155278 A1Published application
System and method for detecting biological materials
AssigneeIndustrial Technology Research Institute
InventorsYi-Chang Chen, Jane SC Tsai
Life of this patent
Estimating the term…
Abstract
A method of identifying a target biological material by using captures and probes. Each capture is specific for a kind of target. Each probe is designed to distinguish the type of the target. The captures and probes are each labeled with one or more detectable labels.
Claims23
A method of identifying a biological material, the method comprising:
- providing a sample containing a biological material, wherein the biological material has a kind and a type;
- adding to the sample one or more captures and one or more probes, each capture and each probe being labeled with one or more detectable labels, wherein each capture is specific for a kind of biological material and each probe is specific for a type of biological material, and the one or more captures are distinguishable from each other by their detectable labels and the one or more probes are distinguishable from each other by their detectable labels, whereby a combination of detectable labels identifies a kind and a type of the biological material;
- allowing the one or more captures and the one or more probes to bind to the biological material;
- and
- determining the detectable labels bound to the biological material, whereby the kind and the type of the biological material are identified.
2 more elements
A method of identifying one or more target nucleic acids, the method comprising:
- providing a sample containing nucleic acid molecules;
- mixing the sample with one or more captures and one or more probes to obtain a mixture, wherein (i) each capture is labeled with one or more detectable labels such that it is distinguishable from the other captures by its detectable labels, and contains a capture nucleic acid sequence complementary to a first target sequence in a target nucleic acid, and (ii) each probe is labeled with one or more detectable labels such that it is distinguishable from the other probes by its detectable labels, and contains a probe sequence that is complementary to a second target sequence in a target nucleic acid, whereby a combination of detectable labels identifies a target nucleic acid molecule;
- allowing the one or more captures and the one or more probes to hybridize to the nucleic acid molecules, thereby complexes each containing a target nucleic acid, a capture, and a probe are formed;
- and
- determining the detectable labels bound to the complexes, whereby one or more target nucleic acids are identified.
2 more elements
Dependencies are inferred from the claim text.