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US 2014/0155277 A1Published application
Adaptive Immunity Profiling and Methods for Generation of Monoclonal Antibodies
AssigneeImdaptive Inc.
InventorSteven R. Wiley
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Abstract
Methods are provided for producing monoclonal antibody candidates using adaptive immunity profiling. In some aspects, the method provides for the use of massively parallel signature sequencing.
Claims24
A method of generating monoclonal antibodies, comprising the steps of:
- a) immunizing a host subject with an antigen;
- b) creating a cDNA sequence library, the creating comprising the steps of:
- i) isolating lymphocytes from the host subject;
- ii) isolating mRNA from the lymphocytes;
- iii) reverse transcribing the mRNA to cDNA;
- iv) amplifying target antibody cDNA sequences;
- and
- v) sequencing the target antibody cDNA sequences;
- and
- c) analyzing the frequency of target antibody cDNA sequences, wherein target antibody cDNA sequences having a relatively high frequency are identified as candidate antibody sequences.
7 more elements
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