Method for conducting platelet aggregation analysis

A method for conducting platelet aggregation analysis, comprising:

• (a) placing a blood sample in a cup portion of a cartridge device, wherein the cartridge device includes at least three electrodes and the at least three electrodes are immersed in the blood sample;
• (b) stirring the blood sample to circulate the blood sample within the cup portion of the cartridge device;
• (c) measuring impedance signals between the at least three electrodes to provide at least a first impedance signal measured between a first one of the at least three electrodes and a second one of the at least three electrodes, a second impedance signal measured between the second one of the at least three electrodes and a third one of the at least three electrodes, and a third impedance signal measured between the first one of the at least three electrodes and the third one of the at least three electrodes;
• (d) comparing the first impedance signal, the second impedance signal and the third impedance signal to a mean or median of the first, second and third impedance signals to determine if a variation for one of the first impedance signal, the second impedance signal and the third impedance signal is higher than a first predetermined threshold;
• (e) discarding one of the first impedance signal, the second impedance signal or the third impedance signal if the variation from the mean or median of first impedance signal, the second impedance signal or the third impedance signal is higher than the first predetermined threshold, respectively;
• (f) comparing a remaining pair of impedance signals of the first impedance signal, the second impedance signal and the third impedance signal if one of the first impedance signal, the second impedance signal or the third impedance signal was discarded in step (e) in order to determine if a variation between two signals comprising the remaining pair of impedance signals is higher than a second predetermined threshold;
• and,
• (1) reporting as a measure of platelet aggregation the first impedance signal, the second impedance signal, the third impedance signal and/or the mean or median value thereof if no impedance signal is discarded in step (e);
• (2) reporting as a measure of platelet aggregation one or more impedance signals of the remaining pair of impedance signals and/or a mean or median value of the remaining pair of impedance signals if the variation of the remaining pair of impedance signals is not determined in step (f) to be higher than the second predetermined threshold;
• or,
• (3) repeating steps (a) through (f) if one of the first impedance signal, the second impedance signal and the third impedance signal was discarded in step (e) and the variation between the remaining pair of impedance signals is determined in step (f) to be above the second predetermined threshold.