US 2014/0154806 A1Published application

Tetraselmis promoters and terminators for use in eukaryotic cells

AssigneeExxonMobil Research and Engineering Company

InventorsJane C. Schneider, Soyan Lieberman, Bo Liu, Eric R. Moellering, John H. Verruto, Amanda Skaggs

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Abstract

The present invention provides novel promoter and terminator sequences for use in gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the gene; and methods for stably transforming eukaryotic algae such as Tetraselmis with transgenes.

Claims37

Dependent claims

An expression cassette comprising a heterologous nucleic acid sequence, operably linked to a promoter comprising a nucleotide sequence selected from the group consisting of a nucleotide sequence having at least 195% identity to at least 100 contiguous nucleotides of SEQ ID NO:1;

a nucleotide sequence having at least 95% identity to at least 100 contiguous nucleotides of SEQ ID NO:2;
a nucleotide sequence having at least 95% identity to at least 100 contiguous nucleotides of SEQ ID NO:3;
a nucleotide sequence having at least 95% identity to at least 100 contiguous nucleotides of SEQ ID NO:4;
a nucleotide sequence having at least 95% identity to at least 100 contiguous nucleotides of SEQ ID NO:5;
a nucleotide sequence having at least 95% identity to at least 100 contiguous nucleotides of SEQ ID NO:6;
a nucleotide sequence having at least 95% identity to at least 100 contiguous nucleotides of SEQ ID NO:7;
and a nucleotide sequence having at least 95% identity to at least 100 contiguous nucleotides of SEQ ID NO:8.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 13/693,585Filed 4 Dec 2012
Published: 5 Jun 2014
Term ends: Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12N 15/8221
Methods for controlling, regulating or enhancing expression of transgenes in plant cells: transit peptides
C07H 21/04
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of…: with…
C12N 15/79
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…
C12N 15/8216
For plant cells , e.g. plant artificial chromosomes: methods for controlling, regulating or enhancing expression of transgenes in plant cells

Family

US 8,883,993 B2Related patent
US 2014/0154806 A1This patent

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