Anti-inflammatory proteins and methods of preparation and use thereof
AssigneeManukaMed Limited
InventorsAmanda Bean, Peter Molan, Ray Cursons, Richard Wilkins
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Abstract
The present disclosure relates to anti-inflammatory proteins, their uses, methods of preparation and methods of their detection. In particular, the invention relates to major royal jelly proteins modified by methyglyoxal and fragments thereof from manuka honey.
Claims33
An isolated apalbumin protein or fragment thereof, which has been chemically modified by methylglyoxal (MGO).
(canceled)
(canceled)
An isolated MGO-modified apalbumin protein or fragment thereof that has anti-inflammatory capacity and at least 85% sequence identity to an amino acid sequence to the amino acid sequence set forth in SEQ ID NO 1.
(canceled)
A method of producing an anti-inflammatory molecule by modifying royal jelly, the method including the step of reacting royal jelly with MGO at a concentration of at least 0.1%, 0.5% or 1.0% MGO.
16-17. (canceled)
A method of identifying (i) the anti-inflammatory capacity or (ii) MGO-modified apalbumin concentration of a sample of honey, comprising the steps of:
- assaying the fluorescence of the honey sample, and
- b) correlating the measure of fluorescence of the honey sample with the anti-inflammatory
- capacity of the honey sample, by comparing the measure of fluorescence of a sample of honey and the anti-inflammatory capacity of one or more samples of honey with previously measured capacity to inhibit phagocytosis.
A method of increasing the anti-inflammatory capacity of one or more apalbumin proteins, by chemically treating the one or more proteins with MGO, formaldehyde, glyoxal and/or glutaraldehyde.
A method of increasing the anti-inflammatory capacity and MGO-modified apalbumin protein content of a sample of honey, comprising the step of adding MGO or a MGO-precursor molecule to a honey sample.
Dependencies are inferred from the claim text.