Endocrine precursor cells, pancreativ hormone-expressing cells and methos of production
AssigneeViaCyte, Inc.
InventorsKevin A. D'Amour, Anne Bang, Emmanuel E. Baetge
Life of this patent
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Abstract
Disclosed herein are cell cultures and enriched cell populations of endocrine precursor cells, immature pancreatic hormone-expressing cells and mature pancreatic hormone-expressing cells. Also disclosed herein are methods of producing such cell cultures and cell populations.
Claims21
1-20. (canceled)
A population of cells expressing markers characteristic of the pancreatic endoderm lineage, wherein said markers are pancreatic duodenal homeobox 1(PDX1) and NKX6 transcription factor related locus 1 (NKX6.1) or pancreas specific transcription factor 1a(PTF1A).
The cell population of claim 1, wherein said population is derived from a human pluripotent stem cell.
The cell population of claim 1, wherein said population is in contact with a gamma secretase inhibitor.
The cell population of claim 1, wherein said population is in contact with a retinoid.
The cell population of claim 1, wherein said population is in contact with noggin.
The cell population of claim 1, wherein said markers are PDX1 and NKX6.1.
The cell population of claim 1, wherein said markers are PDX1, NKX6.1 and PTF1A.
The cell population of claim 1, wherein the population does not substantially express INS or GCG.
An in vitro cell culture comprising pancreatic endoderm cells that express a marker selected from the group consisting of PDX1, NKX6.1 and PTF1A.
An in vitro differentiated population of pancreatic endoderm cells obtained from the differentiation of pluripotent stem cells, wherein the population expresses PDX1 and NKX6.1 or PTF1A.
Dependencies are inferred from the claim text.