Embryonic stem cell line and method for preparing the same

A medium for use in carrying out the in vitro culturing in step (4) of claim 3, comprising:

• 95 to 110 mM NaCl;
• 7.0 to 7.5 mM KCl;
• 20 to 30 mM NaHCO3;
• 1.0 to 1.5 mM NaH2PO4;
• 3 to 8 mM sodium lactate;
• 1.5 to 2.0 mM CaCl2.2H2O;
• 0.3 to 0.8 mM MgCl2. 6H2O;
• 0.2 to 0.4 mM sodium pyruvate;
• 1.2 to 1.7 mM fructose;
• 6 to 10 mg/ml human serum albumin;
• 0.7 to 0.8 μg/ml kanamycin;
• 1.5 to 3% essential amino acids;
• 0.5 to 1.5% nonessential amino acids;
• 0.7 to 1.2 mM L-glutamine;
• and 0.3 to 0.7% a mixture of insulin, transferrin and sodium selenite.