Processing microtitre plates for covalent immobilization chemistries

An article make by a method comprising:

• treating a microtitre plate comprising an organic polymer and comprising a plurality of wells with an electron beam-generated plasma;
• exposing the treated polymer to air or an oxygen- and hydrogen-containing gas;
• wherein the treating and exposing generates hydroxyl groups on the surface of the polymer;
• reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group;
• and
• covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof.