High Fidelity Restriction Endonucleases
AssigneeNew England Biolabs, Inc.
InventorsZhenyu Zhu, Aine Quimby, Shengxi Guan, Dapeng Sun, Yishu Huang, Xuhui Lai, Siu-hong Chan, Xianghui Li, Shuang-Yong Xu, Chunhua Zhang
Life of this patent
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Abstract
Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
Claims4
1-74. (canceled)
A composition comprising a variant BglII restriction endonuclease having reduced star activity, wherein the variant BglII restriction endonuclease comprises an amino acid sequence that differs from the amino acid sequence of the parent BglII restriction endonuclease by an amino acid substitution at one or more positions corresponding to positions selected from 014, 069, 099, 105, 117, 135, and 035 in SEQ ID NO:13.
Dependencies are inferred from the claim text.