High Fidelity Restriction Endonucleases
AssigneeNew England Biolabs, Inc.
InventorsZhenyu Zhu, Aine Quimby, Shengxi Guan, Dapeng Sun, Yishu Huang, Xuhui Lai, Siu-hong Chan, Xianghui Li, Shuang-Yong Xu, Chunhua Zhang
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Abstract
Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
Claims4
1-74. (canceled)
A composition comprising a variant MluI restriction endonuclease having reduced star activity, wherein the variant MluI restriction endonuclease comprises an amino acid sequence that differs from the amino acid sequence of the parent MluI restriction endonuclease by two amino acid substitutions at positions corresponding to positions 112 and 132 of SEQ ID NO:28.
Dependencies are inferred from the claim text.