Stable Lysis Buffer Mixture for Extracting Nucleic Acids
AssigneeSTRATEC Biomedical AG
InventorsPeter Bendzko, Hans Joos
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AssigneeSTRATEC Biomedical AG
InventorsPeter Bendzko, Hans Joos
Estimating the term…
Embodiments relate to a lysis buffer mixture that is stable in storage for isolating nucleic acids from biological, preferably diagnostic samples. The mixture is preferably associated with an extraction control. The aim of the invention is to provide an improved nucleic acid extraction system, which is cost-effective, stable and easy to use, thus fulfilling the requirements of a modern nucleic acid extraction system and containing, among other things, extraction controls. Embodiments relate to a lysis buffer mixture for isolating nucleic acids, said mixture containing non chaotropic salts, a special selection of detergents, a defined quantity of at least one nucleic acid as an extraction control, optionally lytic enzymes, optionally carrier nucleic acids and optionally other additives.
1-28. (canceled)
A method for preparing storage-stable, freeze-dried lysis buffer mixture for the isolation of nucleic acids from arbitrary complex initial substances, wherein said method comprises individual preparation of a first aqueous stem solution and a second aqueous stem solution, which are then mixed with one another, followed by mechanically portioning and freeze drying, wherein said first aqueous stem solution contains a mixture of at least one non-chaotropic salt, Tris, at least one detergent and at least one lytic enzyme, and wherein said second aqueous stem solution contains a nucleic acid for extraction control, carrier nucleic acids, sugar, and Tris-HCl.
Dependencies are inferred from the claim text.