Genes Encoding Z,Z-Farnesyl Diphosphate Synthase and A Sesquiterpene Synthase with Multiple Products and Uses Thereof
OwnerChristophe Sallaud, Denis Rontein +1inventors
InventorsCHRISTOPHE SALLAUD, DENIS RONTEIN, ALAIN TISSIER
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Abstract
The present invention relates to the genes involved in the biosynthetic pathway for sesquiterpenes of SB type (alpha-santalene, epi-beta-santalene, cis-alpha-bergamotene, trans-alpha-bergamotene and endo-beta-bergamotene,) and for the precursor thereof, Z,Z-farnesyl diphosphate (Z,Z-FPP), involving a Z,Z-FPP synthase and a sesquiterpene SB synthase, and to the uses thereof for producing sesquiterpeniques compounds of SB type.
Claims5
A method for producing sesquiterpenes in a cell comprising:
- a) introducing into said cell a construct having an expression cassette comprising a first gene encoding a Z,Z-farnesyl diphosphate synthase (zFPS) having at least 80% sequence identity to SEQ ID NO:
- 2 and a construct having an expression cassette comprising a second gene encoding a SB synthase (SBS) having at least 80% sequence identity to SEQ ID NO:
- 4;
- and
- b) growing the transformed cell in the presence of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) under conditions suitable for the expression of said first and said second genes.
A cDNA encoding a protein having at least 80% sequence identity to SEQ ID NO:
- 2 or having at least 80% sequence identity to SEQ ID NO:
- 4.
A vector comprising a nucleic acid encoding a protein having at least 80% sequence identity to SEQ ID NO:
- 2 or having at least 80% sequence identity to SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.