Enhanced fermentation of cellodextrins and beta-d-glucose

A method of increasing production of a chemical, the method comprising:

• a) providing a host cell, wherein the host cell comprises:
• i) recombinant DNA encoding one or more cellodextrin transporters;
• ii) recombinant DNA encoding one or more polypeptides having glucose mutarotase activity;
• and
• b) culturing the host cell in a medium such that the recombinant DNA encoding the one or more cellodextrin transporters and the one or more polypeptides having glucose mutarotase activity are expressed, and wherein expression of the recombinant DNA encoding one or more polypeptides having glucose mutarotase activity results in increased production of the chemical by the cell as compared with a corresponding host cell lacking said recombinant DNA encoding one or more polypeptides having glucose mutarotase activity.

A method of increasing the growth rate of a cell, the method comprising:

• a) providing a host cell, wherein the host cell comprises:
• i) recombinant DNA encoding one or more cellodextrin transporters;
• ii) recombinant DNA encoding one or more polypeptides having glucose mutarotase activity;
• and
• b) culturing the host cell in a medium such that the recombinant DNA encoding the one or more cellodextrin transporters and the one or more polypeptides having glucose mutarotase activity are expressed, and wherein expression of the recombinant DNA encoding one or more polypeptides having glucose mutarotase activity results in increased growth rate of the cell as compared with a corresponding host cell lacking said recombinant DNA encoding one or more polypeptides having glucose mutarotase activity.