Thermostable chimeric nucleic acid polymerases and uses thereof
AssigneeQiagen GmbH
InventorsDirk Löffert, Andreas Missel, Jie Kang
Life of this patent
Estimating the term…
Abstract
Novel thermostable chimeric nucleic acid polymerases and methods for their generation and use are disclosed. It is shown that these chimeric nucleic acid polymerases, such as DNA polymerases, can be constructed using enzymatically active domains, isolated from different proteins or chemically synthesized. It is demonstrated that chimeric nucleic acid polymerases of the present invention possess the chemical and physical properties of their component domains (e.g., exonuclease activity, thermostability) and that the chimeric polymerases are thermostable.
Claims19
A method of synthesizing a recombinant nucleic acid encoding a thermostable chimeric nucleic acid polymerase having two, non-naturally associated, enzymatically active domains, the method comprising:
- (a) isolating a first nucleic acid encoding a first enzymatically active domain;
- (b) isolating a second nucleic acid encoding a second enzymatically active domain, wherein said second enzymatically active domain is non-naturally associated with said first enzymatically active domain;
- and
- (c) combining said first nucleic acid and said second nucleic acid to form said recombinant nucleic acid encoding said thermostable chimeric nucleic acid polymerase.
Dependencies are inferred from the claim text.