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US 2014/0154743 A1Published application
Methods and materials for enhancing functional protein expression in bacteria
AssigneeXoma Technology Ltd.
InventorsRaphael D. Levy, Chung-Leung Chan, Kiranjit K. Ahluwalia, Toshihiko Takeuchi
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Abstract
Novel materials and methods useful for expressing heterologous proteins in prokaryotic cells are provided, including prokaryotic cells expressing FkpA and/or Skp.
Claims39
A prokaryotic host cell that expresses a heterologous protein for recombinant production comprising:
- (a) a polynucleotide encoding (i) FkpA that is not linked to a functional signal sequence, or a functional fragment thereof, or (ii) Skp that is not linked to a functional signal sequence, or a functional fragment thereof, and
- (b) a polynucleotide encoding the heterologous protein linked to a functional signal sequence.
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A method for increasing recombinant production of a functional heterologous protein in a prokaryotic host cell, comprising co-expressing:
- (a) a polynucleotide encoding (i) FkpA that is not linked to a functional signal sequence, or a functional fragment thereof, or (ii) Skp that is not linked to a functional signal sequence, or a functional fragment thereof, and
- (b) a polynucleotide encoding a heterologous protein that is linked to a functional signal sequence,
- whereby the amount of functional heterologous protein produced is increased compared to expressing (b) in the absence of (a).
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Dependencies are inferred from the claim text.