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AssigneeAnthem Biosciences Pvt Ltd.
InventorsAyyappan Nair, Sunil Kumar Sukumaran, Shalaka Samant, Gunja Gupta, Suthakarn Pichaimuthu, Ganesh Sambasivam
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The present invention relates to a recombinant DNA expression/secretion system in E. coli wherein the said system combines the potential of signal peptide-based translocation of recombinant proteins to the periplasmic space of E. coli with membrane brave defective mutants of E. coli to further aid secretion into the extracellular space. The present invention further relates to the expression system which furthermore includes a helper plasmid to drive the expression of translocons to facilitate improved periplasmic secretion of the over-expressed recombinant protein. In addition, this system also facilitates efficient production of specific proteins of interest in E. coli.
A recombinant host cell comprising an expression vector, optionally along with a helper plasmid, wherein, the said recombinant cell is a membrane defective cell.
A method of obtaining a recombinant cell, said method comprising steps of:
A method of obtaining recombinant peptide, method comprising steps of:
A kit for obtaining a recombinant peptide, said kit comprising an expression vector, a recombinant cell or a combination thereof.
Dependencies are inferred from the claim text.