Duck embryonic derived stem cell lines for the production of viral vaccines
AssigneeValneva SE
InventorsFabienne Guehenneux, Karine Moreau, Magali Esnault, Majid Mehtali
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Abstract
The present invention relates to the development and manufacturing of viral vaccines. In particular, the invention relates to the field of industrial production of viral vectors and vaccines, more in particular to the use of avian embryonic stem cells, preferably the EBx® cell line derived from duck embryonic stem cells, for the production of viral vectors and viruses. The invention is particularly useful for the industrial production of viral vaccines to prevent viral infection of humans and animals.
Claims20
-23. (canceled)
A process for obtaining a continuous avian cell line derived from avian embryonic stem cells (ES), wherein said avian cell line is capable of proliferating in a basal medium in the absence of growth factors, and a feeder layer, said process comprising the steps of:
- a) isolating bird embryo(s) at a developmental stage around oviposition;
- b) suspending avian embryonic stem (ES) cells obtained by dissociating embryo(s) of step a) in a basal culture medium supplemented with:
- Insulin Like Growth Factor 1 (IGF-1) and Ciliary Neurotrophic Factor (CNTF) as the only recombinant growth factors;
- and
- animal serum;
- c) seeding the suspension of ES cells obtained in step b) on a layer of feeder cells and further culturing the ES cells for at least one passage;
- d) withdrawing IGF-1 and CNTF from the culture medium, and further culturing the cells for at least one passage;
- e) progressively decreasing the concentration of feeder cells in the culture medium so as to obtain a total withdrawal of feeder layer after several passages, and further culturing the cells;
- thereby obtaining an adherent continuous avian cell line derived from ES cells capable of proliferating in a basal medium in the absence of growth factors, and a feeder layer.
Dependencies are inferred from the claim text.