Biosensing system with extended lifetime via cofactor recycling

A biosensing system that measures the concentration of an analyte in a solution, said biosensing system comprising

• a first biocomponent that catalyzes the reaction of said analyte and oxygen and uses a cofactor selected from the group consisting of NADH, NADPH, FADH, FADH2, FMNH, FMNH2, and
• a second biocomponent that catalyzes the reaction of a cofactor selected from the group consisting of NAD+, NADP+, FADH, FMNH, FAD and FMN, and an electron donor, and
• wherein said first biocomponent and said second biocomponent are immobilized within a matrix, and
• wherein said matrix is in contact with a transducer layer, and
• wherein said transducer layer is part of an optode.

A method for measuring the concentration of an analyte in a solution using a biosensing system wherein

• said biosensing system comprises biocomponents, and
• wherein a first biocomponent of said biosensing system is an oxygenase enzyme that catalyzes a reaction that uses oxygen, said analyte and a reduced cofactor as reactants and produces oxidized cofactor, and
• wherein a second biocomponent of said biosensing system is a dehydrogenase enzyme that catalyzes a reaction that uses said oxidized cofactor and an electron donor as reactants and produces said reduced cofactor, and
• wherein said reduced cofactor is used in the reaction catalyzed by said first biocomponent, and
• wherein the oxygen concentration in said solution is measured by said biosensing system, and
• wherein the measured oxygen concentration in said solution is used to calculate the measurement of the concentration of said analyte in said solution.