Rapid detection of petroleum in food
AssigneeBioo Scientific Corporation
InventorsJoseph F. Krebs, John Duvall-Jisha, Sol Resnick
Life of this patent
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Abstract
Described herein is a rapid coupled enzymatic assay to detect polycyclic aromatic hydrocarbon (PAH) compounds in samples. The method uses a detection composition that includes: a buffered solution comprising NADH; naphthalene dioxygenase, naphthalene 1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase, and 1,2-dihydroxynaphthalene dioxygenase. The presence of PAH is determined by noting a color change of the detection composition, either visually or spectrophotometrically.
Claims21
A method of detecting polycyclic aromatic hydrocarbons comprising:
- adding a sample suspected of containing one or more polycyclic aromatic hydrocarbons to a detection composition, wherein the detection composition comprises:
- NADH;
- naphthalene dioxygenase;
- naphthalene 1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase;
- and 1,2-dihydroxynaphthalene dioxygenase;
- determining if the color of the detection composition has changed due to the presence of polycyclic aromatic hydrocarbons.
A method of producing stabilized naphthalene dioxygenase comprising:
- obtaining a naphthalene dioxygenase fraction from lysed cells of a bacterial strain which can produce all four subunits of naphthalene dioxygenase;
- dialyzing the obtained naphthalene dioxygenase using dialysis tubing having a molecular weight cut off (MWCO) of less than or equal to about 7500.
A detection composition for detecting the presence of polycyclic aromatic hydrocarbons in a sample, comprising:
- a buffered solution comprising NADH;
- naphthalene dioxygenase,
- naphthalene 1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase, and
- 1,2-dihydroxynaphthalene dioxygenase.
20-24. (canceled)
A test kit to detect polycyclic aromatic hydrocarbons in a sample comprising:
- a buffered solution comprising NADH;
- naphthalene dioxygenase,
- naphthalene 1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase, and
- 1,2-dihydroxynaphthalene dioxygenase.
Dependencies are inferred from the claim text.