Assay of cell mediated immune responsiveness
AssigneeCellestis Limited
InventorsJeff Boyle, Emily Manktelow
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Abstract
This disclosure relates generally to the field of immunological-based diagnostic assays including an assay to measure cell-mediated immunoresponsiveness. The present disclosure teaches determination of the state, progression and/or severity of disease conditions based on a subject's cell-mediated immunoresponsiveness. The assay contemplated herein is capable of integration into standard pathology architecture to provide a diagnostic reporting system and to facilitate point of care clinical management.
Claims16
A method for measuring cell-mediated immune response activity in a subject, said method comprising contacting lymphocytes from the subject with an antigen and a limiting amount of a Toll-like receptor (TLR) agonist and measuring the presence or elevation in the level of an immune effector molecule from immune cells wherein the presence or level of the immune effector molecule is indicative of the level of cell-mediated responsiveness of the subject.
Use of a limiting amount of a TLR agonist in the manufacture of a diagnostic assay of cell-mediated immune responsiveness by the method of incubating limiting amounts of the agonist with lymphocytes and detecting the presence or elevation in effector molecules.
A method of allowing a user to determine the status of cell-mediated immunoresponsiveness of a subject, the method including:
- (a) receiving data in the form of levels or concentrations of an immune effector molecule which relative to a control provide a correlation to the state of cell-mediated immunoresponsiveness from the user via a communications network, the immune effector molecule measured after exposure of lymphocytes to an antigen and a limiting amount of TLR agonist;
- (b) processing the subject data via univariate or multivariate analysis to provide an immunoresponsiveness value;
- (c) determining the status of the subject in accordance with the results of the immunoresponsiveness value in comparison with predetermined values;
- and
- (d) transferring an indication of the status of the subject to the user via the communications network.
Dependencies are inferred from the claim text.