Detection of defensins to diagnose cancer
AssigneesBoard of Trustees of the University of Arkansas, Ascendant Diagnostics, LLC
InventorsAnna Daily, Rohana Liyanage, Jackson O. Lay
Life of this patent
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Abstract
Non-invasive methods of detecting and providing an early diagnosis of cancer, in particular breast cancer are provided herein. The methods include obtaining a sample, suitably an ocular wash sample, from a subject and determining the level of α-Defensin 1, α-Defensin 2 and/or α-Defensin 3 in the sample. Increased levels of any of these proteins as compared to a negative control or non-cancerous subject are indicative of cancer in the subject from which the sample was obtained. Kits for performing the methods described herein are also provided.
Claims21
A method of determining whether a subject is likely to have cancer comprising:
- obtaining a sample from the subject;
- performing steps for or detecting the level of at least one of α-Defensin 1, α-Defensin 2 or α-Defensin 3 in the sample;
- and determining the subject has cancer if the levels of α-Defensin 1, α-Defensin 2 or α-Defensin 3 are increased as compared to the levels in a control sample.
A method of determining whether a subject is likely to have cancer comprising:
- obtaining a sample from the subject;
- preparing the sample for liquid chromatography-mass spectroscopy;
- collecting liquid chromatography-mass spectroscopy spectra of the sample;
- identifying whether the sample contains peaks with increased intensity corresponding to at least one of α-Defensin 1, α-Defensin 2 or α-Defensin 3 in the sample;
- and determining the subject is at increased risk of having cancer if the subject has a peak with increased intensity corresponding to any one of α-Defensin 1, α-Defensin 2 or α-Defensin 3 as compared to the levels in a control sample.
A kit for detecting cancer in a subject, the kit comprising an antibody capable of detecting at least one of α-Defensin 1, α-Defensin 2 or α-Defensin 3 and instructions for performing the assay.
A method of determining whether a subject is likely to have cancer comprising:
- obtaining a sample from the subject;
- performing an antibody based detection assay on the sample to detect the presence and/or level of at least one of α-Defensin 1, α-Defensin 2 or α-Defensin 3 in the sample;
- and determining the subject is likely to have cancer if the levels of α-Defensin 1, α-Defensin 2 or α-Defensin 3 are increased as compared to the levels in a control sample.
Dependencies are inferred from the claim text.