Antibodies to oxidized ldl
OwnerVictor Mannehinventor
Life of this patent
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Abstract
A method is described for obtaining antibodies binding specifically to an epitope resulting from in vivo modification of a biomolecule or biomolecular complex, where the in vivo modification results from expression of a transgene in an animal. An example of such a biomolecule or biomolecular complex is LDL and an example of such a transgene is human myeloperoxidase. Resulting antibodies and methods of using the antibodies are also described.
Claims17
A method for producing antibodies specific for in vivo-modified biomolecules, comprising
- screening monoclonal antibody clones for specific binding activity to an in vivo modification epitope on a biomolecule or biomolecular complex, wherein said antibody clones are derived from a transgenic animal expressing a transgene, wherein expression of said transgene results in said in vivo modification or an increase in the level of said in vivo modification;
- and
- identifying antibody clones having said specific binding activity.
An isolated monoclonal antibody, comprising
- a monoclonal antibody or antibody fragment derived from a transgenic animal expressing a transgene, wherein expression of said transgene results in in vivo modification of biomolecules and said antibody or antibody fragment binds specifically to an epitope containing at least a part of said in vivo modification.
A kit for determining the presence or amount of oxidized LDL (oxLDL) in a sample, comprising
- at least one antibody that specifically binds to an oxidized epitope in oxLDL, wherein said antibody is derived from a transgenic animal expressing human myeloperoxidase and preferentially binds to mpo-LDL rather than un-modified LDL.
A method for determining the presence or amount of oxidized LDL (oxLDL) in a sample, comprising
- contacting said sample with at least one antibody which specifically binds to oxLDL, wherein said at least one antibody is derived from a transgenic animal expressing a transgene which results in in vivo oxidation of LDL;
- and
- detecting binding of said at least one antibody to LDL as an indication of the presence or amount of oxLDL in said sample.
Dependencies are inferred from the claim text.