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US 2014/0154700 A1Published application
Compositions and Methods for Detecting Vitamin D
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsZhu Teng, Roland Janzen, Martin A. Drinan
Life of this patent
Estimating the term…
Abstract
Compounds include carbamate derivatives of vitamin D including vitamin D3 and vitamin D2. The compounds are useful in methods and kits for determining the presence and/or amount of vitamin D including vitamin D analogs and metabolites thereof in a sample suspected of containing the same.
Claims20
A compound of the formula:
- wherein:
- Z is an alkyl, alkenyl or alkynyl group having 1 to 10 carbon atoms, which may be unsubstituted or one or more of which may be substituted by one or more of hydroxy, lower alkoxy, oxo, and oxime;
- R is —O-succinimidyl, —NH—O-(A)k-R2, wherein R2 is a member of a signal producing system, —(CH2)nCOOH, —(CH2)mCOONHS (NHS is N-hydroxysuccinimide), a small molecule, a binding partner for a small molecule, or a support;
- A is a linking group;
- k is 0 or 1;
- n is an integer of 1 to 5 and m is an integer of 1 to 5;
- and
- R4 is H, OH or lower alkoxy.
5 more elements
A compound of the formula:
- or a compound of the formula:
- wherein:
- R′ is —O-succinimidyl or —NH—O-(A)k-R2, wherein R2 is a member of a signal producing system, —(CH2)nCOOH, —(CH2)mCOONHS (NHS is N-hydroxysuccinimide), a small molecule, a binding partner for a small molecule, or a support;
- A is a linking group;
- k is 0 or 1;
- n is an integer of 1 to 5 and m is an integer of 1 to 5;
- R1 is H, OH, or protected OH;
- and
- R4 is H, OH or lower alkoxy.
6 more elements
A compound of the formula:
- or a compound of the formula:
- wherein:
- L is selected from the group consisting of chemiluminescent particles and sensitizer particles and R1′ is OH and A′ is a linking group.
A method of determining in a sample the presence and/or amount of vitamin D, the method comprising:
- (a) providing in combination in a medium:
- (i) the sample,
- (ii) a compound of the formula:
- or a compound of the formula:
- wherein:
- R″ is —NH—O-A″—R2′, wherein R2′ is a member of a signal producing system and A″ is a linking group, and
- R1″ is H or OH;
- and
- (iii) a specific binding member for vitamin D;
- (b) subjecting the combination to conditions for binding of the compound of to the specific binding member to form a complex, and
- (c) measuring the amount of the complex, the amount of the complex being related to the presence and/or amount of vitamin D in the sample.
8 more elements
Dependencies are inferred from the claim text.