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AssigneeQvella Corporation
InventorsSamad TALEBPOUR, Aye Aye KHINE, Robert MAASKANT, Tino ALAVIE
Estimating the term…
Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included, for collecting the microbial cells adjacent to a liquid interface formed by the cushioning liquid, upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed to prior to extraction of the pretreated sample.
A method of extracting microbial cells from a whole blood sample in a pretreatment vessel,
A pretreatment vessel for extracting microbial cells from a whole blood sample, comprising:
Dependencies are inferred from the claim text.