Microfluidic apparatus and method of enriching target material in biological sample by using the same

A method of enriching a target material using a microfluidic apparatus, the method comprising:

• forming a first fluid layer that comprises a first target material and a second fluid layer that comprises a second target material by centrifuging a sample in a sample chamber of the microfluidic apparatus;
• transporting the first and second fluid layers to first and second enriching units, respectively;
• mixing a first fine particle that specifically binds a surface marker of the first target material with the first fluid layer to form a first complex of the first target material and the first fine particle, and separating the first complex from the first fluid layer using a DGM with a density that is less than the density of the first complex but greater than the density of the first fluid layer;
• mixing a second fine particle that specifically binds a surface marker of the second target material with the second fluid layer to form a second complex of the second target material and the second fine particle, and separating the second complex from the second fluid layer using a density difference between the second complex and the second fluid layer;
• and
• transporting the first and second complexes to first and second recovering chambers, respectively.