Micro organ comprising mesenchymal and epithelial cells
AssigneeUniversity of Durham
InventorsAihua Guo, Colin Albert Buchanan Jahoda
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Abstract
The invention provides a micro-organ composite which comprises a core group of cells and an outer layer of cells, wherein the cells of the core group are mesenchymal cells and the cells of the outer layer are epithelial cells or wherein the cells of the core group are epithelial cells and the cells of the outer layer are mesenchymal cells, and wherein the core group of cells is at least partially encapsulated by the outer layer of cells.
Claims31
A micro-organ composite which comprises a core group of cells and an outer layer of cells, wherein the cells of the core group are mesenchymal cells and the cells of the outer layer are epithelial cells, wherein the core group of cells is at least partially encapsulated by the outer layer of cells.
A micro-organ composite which comprises a core group of cells and an outer layer of cells, wherein the cells of the core group are epithelial cells and the cells of the outer layer are mesenchymal cells, wherein the core group of cells is at least partially encapsulated by the outer layer of cells.
The composite according to any one of the preceding claim, wherein the mesenchymal cells and/or the epithelial cells are derived from more than one cell source.
The composite according to any one of the preceding claims, wherein the mesenchymal cells are dermal cells, and wherein the epithelial cells are epidermal cells.
The composite according to any of the preceding claims, wherein said mesenchymal core and epithelial outer layer interact so as to form a basement membrane.
The composite according to any one of the preceding claims, wherein the core group of cells is fully encapsulated by the outer layer of cells.
The composite according to any one of the preceding claim, wherein the composite is in the form of a spherical particulate.
A composite according to any one of the preceding claims for use as a medicament.
A composite according to any one of the preceding claims for use in skin wound healing.
A method of producing a micro-organ cell composite, comprising:
- a) growing disaggregated mesenchymal cells in a hanging drop culture to form an aggregate core of cells;
- and
- b) adding epithelial cells to the aggregate core of cells,
- wherein the epithelial cells grow to form an outer layer on the aggregate core of cells.
A method of producing a micro-organ cell composite, comprising:
- a) growing disaggregated epithelial cells in a hanging drop culture to form an aggregate core of cells;
- and
- b) adding mesenchymal cells to the aggregate core of cells,
- wherein the mesenchymal cells grow to form an outer layer on the aggregate core of cells.
A micro-organ cell composite substantially as described herein with reference to the accompanying drawings.
A method of producing micro-organ cell composite as described herein with reference to the accompanying drawings.
Dependencies are inferred from the claim text.