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US 2014/0154250 A1Published application
Single-chain multivalent binding proteins with effector function
AssigneeEmergent Product Development Seattle, LLC
InventorsPETER ARMSTRONG THOMPSON, JEFFREY A. LEDBETTER, MARTHA SUSAN HAYDEN-LEDBETTER, WILLIAM BRADY
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Abstract
The present disclosure relates to single-chain multivalent binding proteins, compositions comprising the single-chain multivalent binding proteins, and methods of making and using the single chain-multivalent binding proteins.
Claims43
A single-chain multivalent binding protein comprising from amino-terminus to carboxyl-terminus:
- (a) a first binding domain comprising a first light chain immunoglobulin variable region (VL1) and a first heavy chain immunoglobulin variable region (VH1), wherein said variable regions are positioned in a VH1-VL1 or a VL1-VH1 orientation and separated by a first peptide linker of 2-50 amino acids;
- (b) an immunoglobulin hinge domain;
- (c) an immunoglobulin CH2 domain
- (d) an immunoglobulin CH3 domain
- (e) a scorpion linker;
- and
- (f) a second binding domain comprising a second light chain immunoglobulin variable region (VL2) and a second heavy chain immunoglobulin variable region (VH2), wherein said variable regions are positioned in a VH2-VL2 or a VL2-VH2 orientation and separated by a second peptide linker of 2-50 amino acids,
- wherein the multivalent binding protein is capable of forming a dimer in solution.
5 more elements
Dependencies are inferred from the claim text.