Process for separating proteins fibrinogen, factor xiii and biological glue from a solubilized plasma fraction and for preparing lyophilised concentrates of said proteins

A freeze-dried Factor XIII (FXIII) concentrate, obtained by a process comprising the steps of:

• i) equilibrating an anion exchanger of weak base type with a first buffer having a predetermined ionic strength of an alkaline ph,
• ii) loading said anion exchanger of weak base type with a Cohn fraction I, thus allowing the retention of the biological glue,
• iii) eluting the biological glue with a second buffer having an alkaline pH and an ionic strength greater than said first buffer,
• iv) concentrating the biological glue by ultrafiltration, and
• v) separating FXIII from fibrinogen by adding to at least a part of the biological glue eluate at least one chemical agent precipitating the FXIII, wherein the chemical agent precipitating the FXIII is present in the form of an aqueous solution based on sodium citrate and resolubilizing the precipitated FXIII, and
• vi) diafiltering the resolubilized FXIII solution, followed by freeze-drying said solution.