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AssigneeUniversity of Louisville Research Foundation, Inc.
InventorsJanina Ratajczak, Mariusz Z. Ratajczak
Estimating the term…
Methods for purifying very small embryonic-like (VSEL) stem cells from populations of cells suspected of including VSEL stem cells are provided. In some embodiments, the methods include (a) providing a population of cells suspected of including a VSEL stem cell; and (b) isolating a CD45neg/GlyAneg/CD133+/ALDHhigh subpopulation, a CD45neg/GiyAneg/CD133+/ALDlow subpopulation, a CD45neg/Linneg/SSEA-4+/ALDHhigh subpopulation, a CD45neg/Linneg/SSEA-4/AiOlow subpopulation, or any combination thereof from the population, whereby a VSEL stem cell is purified from the population. Also provided are methods for generating in vitro hematopoietic colonies derived from VSEL stem cells and methods for generating lympho-hematopoietic chimerism in a subject.
A method for purifying a very small embryonic-like (VSEL) stem cell from a population of cells suspected of comprising VSEL stem cells, the method comprising:
A method for generating lympho-hematopoietic chimerism in a subject, the method comprising introducing into a lympho-hematopoietic compartment of the subject a plurality of CD45neg/GlyAneg/CD133+/ALDHhigh cells comprising VSEL stem cells, a plurality of CD45neg/GlyAneg/CD133+/ALDHlow cells comprising VSEL stem cells, a plurality of CD45neg/Linneg/SSEA-4+/ALDHhigh cells comprising VSEL stem cells, a plurality of CD45neg/Linneg/SSEA-4+/ALDHlow cells comprising VSEL stem cells, or a combination thereof, wherein the plurality of CD45neg/GlyAneg/CD133+/ALDHhigh cells comprising VSEL stem cells, the plurality of CD45neg/GlyAneg/CD133+/ALDHlow cells comprising VSEL stem cells, the plurality of CD45neg/Linneg/SSEA-4+/ALDHhigh cells comprising VSEL stem cells, the plurality of CD45neg/Linneg/SSEA-4+/ALDHlow cells comprising VSEL stem cells, or the combination thereof were cultured over an OP9 stromal cell feeder layer under conditions sufficient to induce lympho-hematopoietic competency in one or more VSEL stem cells present therein.
Dependencies are inferred from the claim text.