Metal Abstraction Peptide With Release of Metal
AssigneesEchogen, Inc., University of Kansas
InventorsJennifer Ann Stowell Laurence, Mary Elizabeth Krause, George Laurence
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Abstract
Compositions comprising peptides that are capable of binding a metal in a square planar orientation, a square pyramidal orientation, or both, are disclosed. Such compositions can be used for binding and releasing a metal in a variety of contexts and environments, such as the treatment of cancer.
Claims66
A pharmaceutical composition comprising:
- i) a metal-binding peptide, wherein the metal-binding peptide comprises a sequence Z1—XZ3C1Z4C2—Z2, wherein X is any natural or non-natural amino acid or amino acid analogue, and wherein C1 and C2 are each individually chosen from a cysteine and a sulfur-containing alpha or beta amino acid, and wherein Z1, Z2, Z3, and Z4 are each individually a sequence of 1-5 residues, or absent, wherein each residue is independently a natural or non-natural amino acid or analogue thereof;
- ii) a metal bound to the metal-binding peptide;
- and
- iii) a pharmaceutically-acceptable excipient,
- wherein the composition is a unit dosage form.
A method of treating cancer, the method comprising administering to a subject in need or want thereof a therapeutically-effective amount of a composition comprising:
- i) a metal-binding peptide, wherein the metal-binding peptide comprises a sequence Z1—XZ3C1Z4C2—Z2, wherein X is any natural or non-natural amino acid or amino acid analogue, and wherein C1 and C2 are each individually chosen from a cysteine and a sulfur-containing alpha or beta amino acid, and wherein Z1, Z2, Z3, and Z4 are each individually a sequence of 1-5 residues, or absent, wherein each residue is independently a natural or non-natural amino acid or analogue thereof;
- ii) a metal bound to the metal-binding peptide;
- and
- iii) a pharmaceutically-acceptable excipient,
- wherein the composition is a unit dosage form.
A method of providing a metal to a subject in need or want thereof, the method comprising administering to the subject:
- i) a metal-binding peptide, wherein the metal-binding peptide comprises a sequence Z1—XZ3C1Z4C2—Z2, wherein X is any natural or non-natural amino acid or amino acid analog, and wherein C1 and C2 are each individually chosen from a cysteine and a sulfur-containing alpha or beta amino acid, and wherein Z1, Z2, Z3, and Z4 are each individually a sequence of 1-5 residues, or absent, wherein each residue is independently a natural or non-natural amino acid or analogue thereof;
- and
- ii) the metal, wherein the metal is bound to the metal-binding peptide,
- wherein the metal-binding peptide releases the metal in a physiological environment, wherein the release is associated with a decrease in pH.
Dependencies are inferred from the claim text.