Reverse Breeding

A set of primers selected from the group consisting of:

• (SEQ ID NO:
• 9)
• 1.
• 5′ ACAGAGGCTTTTGGGGAATT 3′
• and
• reverse complement primer
• (SEQ ID NO:
• 10)
• 5′ ACAGAGGCTTTTGGGGAATT 3′;
• (SEQ ID NO:
• 13)
• 2.
• 5′ GTTTTTTATGGCTCATATTGGATGTTTYGTNCCNGC 3′
• and
• reverse complement primer
• (SEQ ID NO:
• 14)
• 5′ TCCACAGTATTAGTTCCCTTTCCAWAYTCRTCDAT 3′
• (SEQ ID NO:
• 15)
• 3.
• TgTCCCGGCTGCATCGGCCAAAATCGGC 3′
• and
• reverse complement primer
• (SEQ ID NO:
• 16)
• 5′ GAATTCGTCAATCAAAATCAGTGACCG 3′.

A method for transferring cytoplasmic male sterility (CMS), comprising:

• a) providing a heterozygous starting plant;
• b) allowing the starting plant to produce haploid cells;
• c) creating homozygous plant s from the haploid cells thus obtained;
• and
• d) selecting the plants having the desired set of chromosomes for CMS;
• characterized in that during production of the haploid cells no recombination occurs in order to obtain a limited number of genetically different haploid cells.

A method for making parental lines for the production of F1 hybrid seed, comprising:

• a) providing a heterozygous starting plant;
• b) allowing the starting plant to produce haploid cells;
• c) creating homozygous plants from the haploid cells thus obtained;
• and
• d) selecting the plants having the desired set of chromosomes for the production of F1 hybrid seed;
• characterized in that during production of the haploid cells no recombination occurs in order to obtain a limited number of genetically different haploid cells.