Biomarkers and method for predicting occurence of ventral hernias
AssigneeCurators of the University
InventorsUlus Atasoy, Sharon L. Bachman, Robert Calaluce, Wade Davis, Bruce J. Ramshaw
Life of this patent
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Abstract
Kits, methods of treating, and methods of diagnosing a risk level for an incisional hernia in a subject undergoing abdominal surgery are disclosed. They are designed to determine risk factors for incisional hernia formation based on a subject's unique gene expression profiles.
Claims50
A method of treating an abdominal surgical incision in a subject in need thereof comprising the steps of:
- a. measuring the level of expression of a gene or combination of genes selected from the group consisting of COL1A2, COL3A1, GREM1, and IL10 in a tissue sample obtained from the subject;
- b. comparing the level of expression of the gene or combination of genes in the tissue sample to that in a control sample;
- c. determining a risk level for an incisional hernia in the subject following surgery, wherein the subject has a high risk for an incisional hernia if the level of expression of the gene or combination of genes in the subject's tissue sample displays a greater than 1.5 fold change compared to the level of expression of the gene or combination of genes in the control sample, and a normal risk for an incisional hernia if the level of expression of the gene or combination of genes in the subject's tissue sample displays a 1.5 fold change or less compared to the level of expression of the gene or combination of genes in the control sample;
- and
- d. treating the subject based upon the subject's risk level for an incisional hernia as determined in step c.
A method of diagnosing a risk level for an incisional hernia in a subject following abdominal surgery, comprising the steps of:
- a. measuring the level of expression of a gene or combination of genes selected from the group consisting of COL1A2, COL3A1, GREM1, and IL10 in a tissue sample obtained from the subject;
- b. comparing the level of expression of the gene or combination of genes in the tissue sample to that in a control sample;
- c. determining a risk level for an incisional hernia in the subject following surgery,
- wherein the subject has a high risk for an incisional hernia if the level of expression of the gene or combination of genes in the subject's tissue sample displays a greater than 1.5 fold change compared to the level of expression of the gene or combination of genes in the control sample, and a normal risk for an incisional hernia if the level of expression of the gene or combination of genes in the subject's tissue sample displays a 1.5 fold change or less compared to the level of expression of the gene or combination of genes in the control sample.
A product comprising isolated biomarkers bound to a biochip array, wherein the biomarkers are selected from the group consisting of COL1A2, COL3A1, IL10, and combinations thereof.
A product comprising purified biomarkers bound to a microarray comprising addressable locations using a biospecific capture reagent, wherein the biomarkers are a gene or combination of genes selected from the group consisting of COL1A2, COL3A1, GREM1, and IL10.
A kit for diagnosing a risk level for an incisional hernia in a subject following abdominal surgery, comprising:
- a. purified biomarkers bound to a microarray comprising addressable locations using an adsorbent or capture reagent, wherein the purified biomarkers are selected from the group consisting of COL1A2, COL3A1, GREM1, IL10, or combinations thereof, and
- b. written instructions for diagnosing a risk level for an incisional hernia in a subject following abdominal surgery, comprising the steps of:
- i. measuring the level of expression of a gene or combination of genes selected from the group consisting of COL1A2, COL3A1, GREM1, and IL10 in a tissue sample obtained from the subject;
- ii. comparing the level of expression of the gene or combination of genes in the tissue sample to that in a control sample;
- iii. determining a risk level for an incisional hernia in the subject following surgery,
- wherein the subject has a high risk for an incisional hernia if the level of expression of the gene or combination of genes in the subject's tissue sample displays a greater than 1.5 fold change compared to the level of expression of the gene or combination of genes in the control sample, and a normal risk for an incisional hernia if the level of expression of the gene or combination of genes in the subject's tissue sample displays a 1.5 fold change or less compared to the level of expression of the gene or combination of genes in the control sample.
A biochip array comprising isolated biomarkers, wherein the biomarkers are selected from the group consisting of COL1A2, COL3A1, GREM1, IL10, and combinations thereof.
A biochip array having a plurality of addressable locations, each comprising at least one isolated biomarker, wherein the biomarkers are selected from the group consisting of COL1A2, COL3A1, GREM1, IL10, and combinations thereof.
A product comprising at least one isolated biomarker bound to a bead by a biospecific capture reagent, wherein the biomarkers are selected from the group consisting of COL1A2, COL3A1, GREM1, IL10, and combinations thereof.
A biochip comprising one or more isolated biomarkers, wherein the biomarkers are selected from the group consisting of COL1A2, COL3A1, GREM1, IL10, and combinations thereof.
Dependencies are inferred from the claim text.