Loading patent…
AssigneePresident and Fellows of Harvard College
InventorsKevin C. Eggan, Alexander Meissner, Christoph Bock, Evangelos Kiskinis, Griet Annie Frans Verstappen
Estimating the term…
The present invention generally relates set of reference data or “scorecard” for a pluripotent stem cell, and methods, systems and kits to generate a scorecard for predicting the functionality and suitability of a pluripotent stem cell line for a desired use. In some aspects, a method for generating a scorecard comprises using at least 2 stem cell assays selected from: epigenetic profiling, differentiation assay and gene expression assay to predict the functionality and suitability of a pluripotent stem cell line for a desired use. In some embodiments, the scorecard reference data can be compared with the pluripotent stem cells data to effectively and accurately predict the utility of the pluripotent stem cell for a given application, as well as any to identify specific characteristics of the pluripotent stem cell line to determine their suitability for downstream applications, such as for example, their suitability for therapeutic use, drug screening and toxicity assays, differentiation into a desired cell lineage, and the like.
A method for selecting a pluripotent stem cell line, comprising
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
-44. (canceled)
46-69. (canceled)
70-72. (canceled)
The assay of claim 70, wherein DNA methylation assay is selected from the group consisting of:
The assay of claim 70, wherein the gene expression assay is a microarray assay.
(canceled)
The assay of claim 70, wherein the differentiation assay assess the ability of the pluripotent cell to differentiate into at least one of the following lineages:
77-81. (canceled)
The assay of claim 70, wherein the assay is a high-throughput assay for assaying a plurality of different pluripotent stem cells or induced pluripotent stem cells (iPSCs) from a subject.
83-87. (canceled)
The assay of claim 70, wherein the gene expression assay determines the expression of genes selected from any combination of genes listed in Table 7 or Tables 13A or Table 14.
The assay of claim 70, wherein the DNA methylation assay determines the DNA methylation levels of any combination of a plurality of target genes selected from the group listed in Table 12A or Tables 13A or Table 14.
-95. (canceled)
The assay of claim 70, wherein the gene expression assay determines the gene expression level of any combination of a plurality of target genes selected from the group listed in Table 12B or Tables 13A or Table 14.
-133. (canceled)
A scorecard of the performance parameters of a pluripotent stem cell, the scorecard comprising:
(canceled)
137-147. (canceled)
-233. (canceled)
Dependencies are inferred from the claim text.