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OwnerHongfang Lu, Andrew Chwee Aun Wan +2inventors
InventorsHongfang Lu, Andrew Chwee Aun Wan, Jackie Y. Ying, Karthikeyan Narayanan
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The present invention relates to a method of releasably encapsulating pluripotent embryonic stem cells in a degradable continuous polyionic fiber for tissue culture, wherein the encapsulated embryonic stem cells are able to maintain a pluripotent phenotype in tissue culture; the method comprising (a) contacting an aqueous solution of a polyanion with an aqueous solution of a polycation to form an interface between the aqueous solution of polyanion and the aqueous solution of polycation, and wherein the aqueous solution of polyanion or the aqueous solution of polycation or both the aqueous solution of polyanion and the aqueous solution of polycation comprises a suspension of pluripotent embryonic stem cells; (b) drawing a continuous polyionic fiber which comprises encapsulated pluripotent embryonic stem cells from the interface; (c) passing the continuous polyionic fiber comprising encapsulated pluripotent embryonic stem cells in a continuous process through a solution which reduces secondary complexation of the components of the polyionic fiber.
A method of releasably encapsulating pluripotent embryonic stem cells in a degradable continuous polyionic fiber for tissue culture, wherein the encapsulated embryonic stem cells are able to maintain a pluripotent phenotype in culture;
A method of releasing encapsulated human pluripotent embryonic stem cells from a continuous polyionic fiber which comprises chitin and alginate, the method comprising exposing the polyionic fiber comprising encapsulated cells to chitinase or to chitinase and alginate lyase simultaneously and/or sequentially to degrade the polyionic fiber sufficiently to allow the release of the encapsulated cells from the polyionic fiber.
An assembly for maintaining pluripotent embryonic stem cells in culture, comprising a degradable continuous polyionic fiber comprising releasably encapsulated pluripotent embryonic stem cells, wherein the polyionic fiber is held by a fiber holder, and wherein the assembly is of neutral buoyancy or is submersible in culture medium, wherein the polyionic fiber is formed from a polyanionic solution comprising alginate and a polycationic solution comprising chitin and/or chitosan.
Dependencies are inferred from the claim text.