Epigenomic induced pluripotent stem cell signatures
AssigneeThe Salk Institute for Biological Studies
InventorsRonald M. Evans, Joseph R. Ecker, Ryan Lister
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Abstract
Provided herein are methods of characterizing the epigenetic signature of human induced pluripotent stem cells. The methods are useful in identifying human induced pluripotent stem cells (hiPSCs), diagnostic markers for incomplete hiPSCs reprogramming, and characterization of the efficacy of different reprogramming techniques.
Claims20
A method of identifying a human induced pluripotent stem cell, said method comprising identifying a non-CpG hypomethylated DMR within said human induced pluripotent stem cell.
A method of identifying a human induced pluripotent stem cell, the method comprising identifying a hypomethylated CG-DMR, or a hypermethylated CG-DMR within said human induced pluripotent stem cell.
A method of identifying a human induced pluripotent stem cell, comprising:
- (i) determining a methylation pattern of at least a portion of a subject cell;
- and
- (ii) comparing said methylation pattern to a human embryonic stem cell methylation pattern, wherein a difference in methylation pattern is indicative of said subject cell being a human induced pluripotent stem cell.
Dependencies are inferred from the claim text.