Live attenuated catfish vaccine and method of making
AssigneeMississippi State University
InventorsMark L. Lawrence, Attila Karsi
Life of this patent
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Abstract
A high throughput bioluminescence mutant screening procedure is disclosed. This procedure utilizes robotics, and bacterial luciferase to allow real-time monitoring of mutant viability. The procedure was used to decelop a live attenuated vaccine for a catfish against E. ictaluri, which is further claimed herein. Additionally, genes from other bacterial species are disclosed which may also be used to create vaccines.
Claims10
A composition for providing immunological protection from diseases caused by Edwardsiella ictaluri which comprises a live attenuated strain of bacteria, wherein said live attenuated strain of bacteria is selected by introducing random transposon mutations into bacterial strains expressing bacterial luciferase operon and screening for said mutations by measuring reduction in bioluminescence.
A composition for providing immunological protection from diseases caused by Edwardsiella ictaluri which comprises a live attenuated strain of the bacteria selected from the list of mutations consisting of:
- EiAKMut01, EiAKMut02, EiAKMut03, EiAKMut04, EiAKMut05, EiAKMut06, EiAKMut07, EiAKMut08, EiAKMut09, EiAKMut10, EiAKMut11, EiAKMut12, EiAKMut13, and EiAKMut14.
A live attenuated vaccine prepared comprising a composition created by introducing random transposon mutations into bacterial strains expressing bacterial luciferase operon and screening for said mutations by measuring reduction in bioluminescence.
A composition for providing immunological protection from diseases caused by Edwardsiella ictaluri which comprises a live attenuated strain of the bacteria having at least one mutation in the genes coding for glycine cleavage system (gcvP), serine hydroxymethyltransferase, succinate dehydrogenase, malate dehyrogenase, 2-oxoglutarate dehydrogenase, negative regulator of sigma E activity (rseB), hypothetical protein pEI1_p1, electron transport complex protein RnfB, Fimbrial chaperon protein, Putative RNA one modulator protein pEI1_p4, or UDP-glucose 6-dehyrogenase, fumarate reductase (frdA), and genes encoding enzymes in the tricarboxylic acid (TCA) cycle.
A composition for providing immunological protection from diseases caused by bacteria which comprises a live attenuated strain of the bacteria having at least one mutation in the genes coding for glycine cleavage system (gcvP), serine hydroxymethyltransferase, succinate dehydrogenase, malate dehyrogenase, 2-oxoglutarate dehydrogenase, negative regulator of sigma E activity (rseB), hypothetical protein pEI1_p1, electron transport complex protein RnfB, Fimbrial chaperon protein, Putative RNA one modulator protein pEI1_p4, or UDP-glucose 6-dehyrogenase, fumarate reductase (frdA), and genes encoding enzymes in the tricarboxylic acid (TCA) cycle.
Dependencies are inferred from the claim text.