Multianalyte molecular analysis using application-specific random particle arrays
AssigneeBioArray Solutions, Ltd.
InventorsSukanta Banerjee, Michael Seul, Alice X. Li
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Abstract
The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interations of a number of analyte molecules in a sample.
Claims22
A method comprising:
- providing an array of particles on a chip, wherein the array comprises two or more particle populations, each population having an optically distinguishable signature and each population displaying a different capture moiety capable of binding to a different analyte, wherein at least one population displays a DNA fragment and at least one population displays a protein;
- contacting the array of particles with a first solution under conditions permitting the capture of an analyte in the first solution by the protein, wherein an optical signal is generated following the capture;
- imaging the optically distinguishable signatures associated with the particles, and correlating the optical signals with particles having particular optically distinguishable signatures to determine that analyte is bound by the protein;
- contacting the array of particles with a second solution under conditions permitting the capture of an analyte in the second solution by the DNA fragment, wherein an optical signal is generated following the capture;
- and
- imaging the optically distinguishable signatures associated with the particles and the optical signals, and correlating the optical signals with particles having particular optically distinguishable signatures to determine that analyte is hybridized by the DNA fragment.
An array of particles on a chip, wherein the array comprises a plurality of two or more particle populations, each population having an optically distinguishable signature and each population displaying a different capture moiety capable of binding to a different analyte, wherein at least one population displays a DNA fragment and at least one population displays a protein.
Dependencies are inferred from the claim text.