Systems for Factor VIII Processing and Methods Thereof
AssigneeBiogen Idec Hemophilia Inc.
InventorsRobert T. Peters, Susan C. Low
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Abstract
The present invention provides methods of reducing nonprocessed Factor VIII or a chimeric polypeptide comprising Factor VIII comprising co-transfecting in a host cell a polynucleotide encoding Factor VIII with a polynucleotide encoding a protein convertase, where the endogenous processing enzymes of the host cell are insufficient to convert all of the Factor VIII to its processed isoform; expressing a proprotein convertase from a second polynucleotide in the host cell; and reducing the nonprocessed Factor VIII by processing with said proprotein convertase.
Claims49
A method for decreasing nonprocessed Factor VIII in a culturing medium, comprising,
- a) expressing Factor VIII from a first polynucleotide in a host cell, where the endogenous processing enzymes of the host cell are insufficient to convert all of the Factor VIII to its processed isoform;
- b) contacting said Factor VIII with an exogenous proprotein convertase;
- and
- c) decreasing the nonprocessed Factor VIII by processing with said proprotein convertase.
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A host cell comprising an expression vector comprising a first polynucleotide sequence encoding Factor VIII and a second polynucleotide sequence encoding a proprotein convertase, wherein said Factor VIII is processed by said proprotein convertase.
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An expression vector comprising a first polynucleotide encoding Factor VIII and a second polynucleotide encoding a proprotein convertase, wherein said Factor VIII is processed by the proprotein convertase when said Factor VIII and said proprotein convertase are expressed in a host cell comprising said vector.
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Dependencies are inferred from the claim text.