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AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsLeaf HUANG, Xiang GAO, Frank L. SORGI
Estimating the term…
Novel stable, concentrated, biologically active and ready-to-use lipid-comprising drug delivery complexes and methods for their production are described. The biological activity of the complexes produced are comparable to the formulations prepared according to the prior art admixture method and upon purification, the complexes produced by the method of this invention are 50 to 500 fold more concentrated than the complexes formed by admixture. The method described herein provides for the large scale production of lipid-comprising drug delivery systems useful for gene therapy and other applications.
-28. (canceled)
A noncovalent nucleic acid/lipid/polycation complex having a net positive charge at pH 6.0-8.0 comprising at least one cationic lipid and a polycation in a ratio of nucleic acid to lipid to polycation which results in the production of said complexes, said polycation selected from the group consisting of polyarginine, polyornithine, polybrene (hexadimethrine bormide), histone, cationic dendrimer, spermine, spermidine, protamine, and synthetic peptidesderived from SV40 large T antigen and comprising a nuclear localization sequence.
A method for producing a noncovalent nucleic acid/lipid/polycation complex having a net positive charge at pH 6.0-8.0, the method comprising, mixing said nucleic acid with at least one cationic lipid and polycation in a ratio of nucleic acid to lipid to polycation which results in the production of said complexes, said polycation being selected from the group consisting of polyarginine, polyornithine, polybrene (hexadimethrine bormide), histone, cationic dendrimer, spermine, spermidine, protamine, and a synthetic peptide comprising a nuclear localization sequence of SV40 large T antigen.
Dependencies are inferred from the claim text.