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US 2013/0281308 A1Published application
Methods for sorting nucleic acids and preparative in vitro cloning
AssigneeGen9, Inc.
InventorsLi-yun A. Kung, Joseph Jacobson
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Abstract
Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids using high throughput sequencing. Specifically, nucleic acid molecules having the desired predetermined sequence can be sorted from a pool comprising a plurality of nucleic acids having correct and incorrect sequences.
Claims12
A method of sorting nucleic acid molecules having a predetermined sequence, the method comprising:
- (a) contacting a pool of nucleic acid molecules comprising at least two populations of nucleic acid molecules, each population of nucleic acid molecule having a unique nucleic acid sequence;
- (b) tagging the 5′ end and the 3′ end of the nucleic acid molecules with an oligonucleotide tag sequence, wherein the oligonucleotide tag sequence comprises a unique nucleotide tag and a primer region;
- (c) subjecting the nucleic acid molecules to a sequencing reaction from both ends to obtain a paired end read;
- and
- (d) sorting the nucleic acid molecules having the predetermined sequence according to the identity of their corresponding unique nucleotide tags.
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Dependencies are inferred from the claim text.