Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to toxic molecule

A method for the selection of recombinant clones, said method comprising:

• obtaining a prokaryotic host cell comprising in its genome a nucleotide sequence encoding a poison protein;
• and
• introducing into said host cell a DNA insert and a nucleotide sequence encoding a functional antidote protein to the poison protein, wherein said recombinant clones are the ones which survive following introduction of said DNA insert and said nucleotide sequence encoding the functional antidote protein to the poison protein into the host cell,
• wherein the antidote and poison proteins are selected from the group consisting of the following coupled proteins:
• CcdA/CcdB protein, K is/Kid protein, Phd/Doc protein, RelB/relE protein, PasB/PasA protein, PasC/PasA protein and mazE/mazF protein.