Extraction of nucleic acids from wax-embedded samples
AssigneeQiagen GmbH
InventorsMarkus Mueller, Thorsten Singer, Esther Rosenberg, Joerg Hucklenbroich
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for extracting nucleic acids from a wax-embedded sample, the use of particular solvents for removing wax from a wax-embedded sample in a method for extracting, isolating and/or purifying nucleic acids from a crosslinked wax-embedded sample as well as to a kit for extracting, isolating and/or purifying nucleic acids from a wax-embedded sample.
Claims38
1-15. (canceled)
A method for extracting nucleic acids from a wax-embedded sample, comprising:
- (1) contacting the sample with a wax-solubilizing agent comprising at least one organic solvent immiscible with water,
- (2) optionally incubating the mixture obtained in step (1),
- (3) adding an aqueous lysis buffer to the mixture still comprising the wax-solubilizing agent, and
- (4) incubating the mixture obtained in step (3) to obtain a bi- or multi-phasic mixture, comprising at least an organic phase, said organic phase consisting essentially of the dissolved wax and the wax-solubilizing agent, and an aqueous phase, said aqueous phase comprising the nucleic acids, wherein steps (1) and (3) may as well be carried out as one combined step.
A method for extracting, isolating, and/or purifying nucleic acids from a cross-linked wax-embedded sample, comprising:
- lysing the cross-links in the sample in a bi- or multi-phasic mixture that comprises (a) one or more solvents selected from the group consisting of linear, branched, and cyclic C10-C16 alkanes and mixtures thereof, and (b) an aqueous lysis buffer.
Dependencies are inferred from the claim text.